METHOD:PF4 complementary deoxyribonucleic acid (cDNA) was amplified by polymer ase chain reaction (PCR), then cloned into pUC19 vector. After sequencing,PF4-c DNA was subcloned into eukaryotic expression vector pIRES2-EGFP.

• 方法:人工合成PF4基因模板,通过PCR方法扩增PF4-cDNA,然后克隆至pUC19克隆载体,经序列测定后重组入pIRES2-EGFP真核表达载体并进行酶切鉴定。