METHOD VSMC were labelled with fluo 3 AM, a calcium sensitive fluorescent dye, and [Ca 2+ ] i was determined by the use of the adherent cells analysis and sorting apparatus(ACAS 570).

• 方法 用荧光染料Fluo 3 AM标记细胞 ,在粘附式细胞仪上测定细胞内游离钙的变化。