MBD1 siRNAs had beensuccessfully integrated into the plasmid. MBD1 siRNAs vector was transfected intopancreatic cancer cell AsPC-1 by liposome. Positive clone was obtained by the screenof G-418.

• 采用脂 质 体 介 导 的 方 法 将 MBD1siRNAs 表 达 质 粒 转 染 胰 腺 癌 细 胞 系AsPC-1,通过 G-418 的筛选获得稳定表达 MBD1siRNAs 的阳性克隆。