Lung of eight animals in each group was immediately resected for determination of SP-A mRNA expressionby Reverse transcriptase-polymerase chain reaction analysis(RT-PCR) and Na+-K+-ATP ase activity was examined by biochemical assay.

• 取每组8只大鼠肺组织,生化方法测定Na+-K+-ATP酶活性,RT-PCR测定肺组织SP-AmRNA蛋白表达;