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Liquid fermentation for nattokinase by Bacillus subitilis natto BSN was investigated. The components of its culture medium including carbon source,nitrogen source,the ratio of carbon source to nitrogen source and ion composition were studied.
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以纳豆枯草芽孢杆菌(Bacillus subitilis natto)为出发菌株,对其液体发酵生产纳豆激酶(Nattokinase,NK)的培养基组成(碳源、氮源、碳氮比和金属离子组成)和培养条件(温度、pH值,发酵时间、接种量和装液量)对产酶量的影响进行了研究。
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