Keywords preimplantation genetic diagnosis;nested-polym erase chain reaction;whole genome amplification;primer extension preamplific ation;embryo biopsy;thalassemia;

• 植入前遗传学诊断;巢式-聚合酶链反应;全基因组扩增;扩增前引物延伸法;胚胎活检;地中海贫血;