In the buffering solution of Na_(2)B_(4)O_(7)-HCl,copper reacted with CAS(chrome axurol S) indicating color with in the presence of CTMAB(hexadecyl-trimethylammonium bromid),a Kelly substance was made,then determined in 605 nm.

• 在硼砂-盐酸缓冲溶液中,铜与铬天青S以及溴化十六烷基三甲基铵(CTMAB)反应生成黄绿色的络合物,在605 nm波长下可以检测。