In situ hybridization technique was used to study the effects of PDGF BB polypeptied on the expression of PDGF A and PDGF B mRNA in cultured monolayer porcine pulmonary artery smooth muscle cells(PASMC).

• 应用地高辛（Digoxigenin）标记的血小板源性生长因子（PDGF）?A和PDGF?B链cDNA探针，原位检测了PDGF?BB多肽对单层培养的肺动脉平滑肌细胞PDGF基因表达的影响。