In 1972 , Dutch De Vessels split pleat bacterium protoplasm body by lyase for the first time. The protoplast of Agaricus bisporus and straw mushroom were split soon.

• 1972 年荷兰De Vessels首次用裂解酶分离了裂褶菌原生质体,随后又分离了双孢蘑菇和草菇的原生质体。