Image collection and analysis: using software of Melanie 3.0. a) Illustrate the difference of electrophoresis between one-step procedure and sequential extraction procedure; b) Evaluate the repeatability of the electrophoresis.

• 图象采集与分析：将双向电泳图谱输入计算机，使用Melanie3.；0双向电泳图象分析软件分析，比较分步提取法与一步法差异：以正常大肠粘膜组织行对照、斑点配比，评价双向电泳重复性。