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Human lung cancer cells (ASTC a 1) were infected with EGFP plasmid vectors, and incubated in RPMI 1640 culture medium supplemented with 15% FCS and 800 mg/L G418. Strong fluorescence of five cell clones were observed after several generations.
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以绿色荧光蛋白 (GFP)作为标记基因转入人类肺癌细胞系 (ASTC a 1) ;经 80 0mg/LG4 18筛选 ;获得5株高表达细胞系 .
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