HER 2/neu receptor ligand domain 2 (RLD2) cDNA was amplified by PCR, cloned into the prokaryotic expression vector containing thioredoxin A (TrxA) gene and expressed in soluble form as TrxA RLD2 fusion protein.

• 用PCR技术扩增HER 2 neu胞外配体结合区 2 (RLD2 )cDNA ；并将扩增的基因片段克隆于硫氧还蛋白 (TrxA)原核表达载体中 ；获得TrxA RLD2融合蛋白的可溶性表达 .