G3P DHG was purified 123.6-fold by gel filtration on Sephadex G-25, affinity chromatography on blue Sepharose and ion-exchange chromatography on Mono Q.

• 粗酶液经Sephadex G-25凝胶过滤；Blue Sepharose亲和层析以及Mono Q离子交换等步骤；提纯123.；6倍；得纯酶液。