Further- more,the expressions of Pro-caspase-3,Bcl-2 and the location of AIF in cells(mitochondria,cyto- plasm or nuclei)were tested by western blot and immunofluorescence assay.

• 应用Western blot法和荧光免疫法测定Caspase-3酶原、Bcl-2蛋白的变化以及检测AIF蛋白在细胞中的定位。