For much more sequence coverage, higher identification score and accuracy, some pretreating process must be carried out before protein identification by database searching.

• 因此,必须采取合适的数据的预处理方法和搜索策略及搜索鉴定结果的优化策略,以提高肽序列鉴定得分,从而提高蛋白鉴定的覆盖率和准确度。