Expression of Fas was analyzed by a FACScan flow cytometry. Transcription and mutation of Fas gene were detected by RT PCR and single stranded conformation polymorphism(SSCP) analysis.

• 用流式细胞仪检测细胞表面Fas的表达 ,用RT PCR结合单链多态构象性分析 (SSCP) ,探讨Fas基因的转录及可能存在的Fas基因突变。