Exect gene setuence hVEGF165 was obtained from the fetal human myocardium tissue by RT PCR; pcDNA3.1/VEGF165 was constructed ; and transient expression was going after transfecting COS 7 cell.

• RT PCR方法从胎儿心肌组织获得正确的hVEGF165基因序列,成功构建pcDNA3·1 /hVEGF165且实现转染COS 7细胞的瞬时表达。