Every group was randomly divided into three subsection 12h,24h,72h group after the injection. Evans blue was injected via cervical vein to five guinea pigs in every subsection 2h before sacrificed.

• 每组按12h、24h、72h三个时相点分组,各组取5只(10耳)在各时相点前2小时颈静脉注射伊文思蓝(Evans Blue,EB),生理盐水心脏灌注冲洗至右心耳流出淡红色液体后,快速处死动物解剖出骨性耳蜗,置于甲酰胺液内,取浸出液检测伊文思蓝通过血迷路屏障的渗出量的动态变化。