Enzyme-linked immunosorbent assay was adopted to detect the concentrations of Serum E2, FSH, LH and PRL.

• 采用酶联免疫吸附法检测治疗前后血清雌二醇(E2)、卵泡刺激素(FSH)、黄体生成素(LH)以及催乳素(PRL)浓度。