Enro was coupled with carrier protein bovine serum album(BSA) and ovalbumin (OVA) to prepare the immune antigen Enro-BSA and the detection antigen Enro-OVA by N-hydroxysuccinimide ester (NHS) method respectively.

• 首先以牛血清白蛋白(BSA)为载体蛋白,与Enro 偶联合成免疫抗原Enro-BSA; 同样的方法,以卵清白蛋白(OVA)为载体蛋白,使之与Enro 偶联合成检测抗原Enro-OVA,并且进行紫外扫描光谱、动物实验及偶联比计算等方面的鉴定。