Decalcification of the cochleae was performed in 100 g/L EDTA solution for 1 week. Then the specimens were placed in 250 g/L sucrose solution overnight and embedded in OCT.

• 100g/L乙二胺四乙酸溶液脱钙1周后,移入250g/L蔗糖溶液中过夜沉淀。