DMF is used as the coupling solvent; Piperidine-DMF to removed the Fmoc group; TBTU/HOBt/DIEA as the activation system,and TFA/EDT/TIS/H_2O as the cleavage condition.

• 以DMF为反应溶剂；Piperidine-DMF脱去Fmoc保护基团；以TBTU/HOBt/DIEA作为肽键缩合试剂进行接肽反应；用TFA/EDT/TIS/H2O定量地从树脂上切除；制备型高效液相色谱分离纯化粗肽.