Control group was set up in each of these three stages. Every pregnant mouse of treated and control groups in the three stages was given the dose of 1/30 LD50 toosendanin and same amount of PBS 2 times by ip injection respectively.

• 每个时期均设对照组，以小鼠腹腔注射半数致死量（LD50）1/30的川楝素剂量对以上3个时期实验组孕鼠染毒2次，对照组孕鼠用等量PBS液注射；