CFs number was measured by MTT assay. Collagen content of CFs culture supernatant was estimated by hydroxyproline chromatogragy. Nitrate enzyme reverting method was adopted to evaluate NO content.

• 方法采用胶原酶消化法培养Wistar大鼠的CFs、四氮唑蓝(MTT)比色法测定CFs的增殖情况、羟脯氨酸比色法测定CFs培养上清胶原含量、硝酸还原酶法测定CFs培养上清NO含量、分光光度计法测定CFs培养上清NOS活性。