C57BL/6 mice were immunized intramuscularly every 2 weeks for 3 times with pcDNA3.0-backbone recombinant plasmids of Hantaan virus 84Fli that encoded the NP and GP proteins,and contrasted with bivalent inactivated EHF vaccine.

• 为了加强我国病毒性出血热的防治；本研究将汉滩病毒84Fli株核蛋白S和糖蛋白M编码片段分别克隆至pcDNA3.；0载体；构建了pcDNA3/84S和pcDNA3/84M重组质粒；等量混合采用肌肉注射途径免疫C57BL/6小鼠；免疫3次；每次间隔2周；同时与双价出血热病毒灭活疫苗进行对比。