C2C12 cells were commonly cultured in DMEM-F12 medium that contained 100 mg/L penicillinum,100 mg/L phytomycin and 10% fetal cattle serum(FCS).

• C2C12细胞置于含100mg/L青霉素；100mg/L链霉素和体积分数为0.；1胎牛血清的DMEM-F12培养基中常规培养。