By the BP recombination reaction,the PCR product containing attB was transfered to an attP-containing donor vector to create an entry clone. Finally,DREB1A gene was shutted into pH2GW7 vector by LR recombination reaction.

• 通过BP反应将包含有attB接头的PCR产物克隆到含有attP的donor载体上以产生Entry克隆,通过LR反应将已经重组入Entry载体的DREB1A基因再克隆到pH2GW7双元载体。