By screening polymorphism between two parents with 500 Operon RAPD 10-mer-primers (25 sets), 462 primers produced clear bands, whichaccounted for 92.4%.

• 利用DH群体两亲本筛选能产生多态性的RAPD引物，筛选了25套500个Operon系列的10碱基引物，其中462个引物可扩增到清晰的条带，有效引物的比例为92.；4%25，谱带总数为2032条，平均每个引物扩增的谱带数为4