Both RT PCR and immunofluorescent staining of recombinant plasmid transfected COS 7 showed positive reaction, thus indicating that the recombinant plasmid pcDNA3 LACK can express LACK protein in euka ryotic cell COS 7.

• 实验结果显示转染了重组质粒的 COS- 7细胞 ,其 RT- PCR及免疫荧光检测均呈阳性反应 ,证实重组质粒 pc DNA3-L ACK能在 COS- 7细胞中有效表达 L ACK蛋白。