At last, above sample can be digested by enterokinase(yeast type) successfully to remove the extra N-peptide and showed the bioactivity was about 513U/mg.

• 本文最后对酶切方法与活性检测方法进行了简单研究，发现酵母型肠激酶可以对目标蛋白进行比较成功的酶切，酶切后的重组人溶菌酶经热激法在电镜下微观观察有较明显的杀菌作用，且经溶壁微球菌比浊法测得重组人溶菌酶的平均质量酶活性为513.