And cloning to the expression vector pET-32a(+) fused with Trx-Tag coding sequence or not, to acquire the recombinant plasmid trx-fnb- pET-32a(+), trx-cna- pET-32a(+),fnb- pET-32a(+) and cna- pET-32a(+).

• 并克隆于原核表达载体pET-32a(+)，分别构建了融合基因表达质粒trx-fnb-pET-32a(+)，trx-cna-pET-32a(+)和非融合基因表达质粒fnb-pET-32a(+)，cna-pET-32a(+)；