Also, with frozen, semen should always be put into the uterus using surgical insemination or insemination by rigid endoscopy (TCI, Trans Cervical Insemination).

• 另外，冷冻精子需要用外科用受精设备，严格采用内窥镜检查法（TCI，子宫颈受精法）将之注入母犬子宫内。