Aim To investigate the mRNA levels of tissue inhibitor of metalloprot einase-1(TIMP-1),tissue inhibitor of metalloproteinase-2 (TIMP-2) in rat car diac fibroblasts by multiplex reverse transcription-polymerase chain reaction ( RT-PCR).

• 目的通过建立多重反转录聚合酶链反应(RT-PCR)方法,旨在测定大鼠心肌成纤维细胞组织金属蛋白酶抑制剂-1(TIMP-1)、组织金属蛋白酶抑制剂-2(TIMP-2)的mRNA水平。