After coculture for 24 h, the proliferation of HSCs was inhibited to a moderate extent by MSCs. With the coculture time lasted, the significant growth suppression of HSCs was affected by MSCs (15.7% and 30.3%) but not BRL cells in 48 h and in 72 h.

• HSCs与MSCs共培养24h；HSCs表现轻度增殖抑制；随着培养时间延长；HSCs增殖活性受抑制更明显；在48h和72h的抑制率分别达15.；7%25与30