After chromosome DNA was digested by Sau3A, chromosomeDNA fragments were ligated to Sau3A linker-adaptor, then amplified by two roundPCR PCR preducts were enough to construct a library of specific chromosome.

• 染色体DNA经Sau3A酶切后与人工合成的Sau3A连接接头连接，经两次PCR扩增获得足以用于构建单染色体DNA文库的扩增产物。