After affinity chromatography, SDS-PAGE showed only one clear band existed in 125 mmol/L or 250 mmol/L imidizone elution.

• 诱导3h的菌体超声破碎后,Ni柱亲和层析,125、250mmol/L咪唑洗脱液SDSPAGE电泳显示出清晰的单一条带。