After T cells being activated by PHA or by anti-CD3 with anti-CD28 mAbs, the GL50 and ICOS expressions on activated T cells were detected by means of FACS on Oh, 24h, 48h, 72h, and 96h.

• 将人外周血T细胞用PHA或联合抗CD3和抗CD28单抗激发后，分别于0h、24h、48h、72h、96h用免疫荧光标记和流式细胞术分析，检测细胞表面GL50分子和ICOS分子的动态表达，结果显示：GL50分子的表达在T细胞活化后24h即开始上调，72h时达到高峰；