According to published HN protein gene sequence of NDV, we designed and synthesiezed a pair of primers. Genetic RNA of NDV B (95)strain, which was isolated from Brisbane in Australia, was used as template from cDNA synthesis of HN gene.

• 根据 NDV HN蛋白已知基因序列设计一对引物 ；以提取的澳大利亚布里斯班 NDV分离株 B95基因组 RNA为模板 ；利用 RT- PCR技术扩增得到一条约 1.；9kb的条带。