According to NHS and MA methods, TS hapten was cross linked to BSA (OVA).Artificial immunogen and coating were synthesized.UV-scanning was used for preliminary qualitation of conjugates.

• 采用混合酸酐法、碳二亚胺法合成人工免疫原、包被原，通过紫外扫描法对其偶联结果进行初步鉴定，结合免疫小鼠及新西兰白兔的试验，间接竞争ELISA监测表明多抗血清针对磺胺类药物产生特异性抗体，确证偶联成功。