About 600bp DNA (p-CpG-DNA) which contained 62 CpG motifs was obtained by the method of plasmid recombination and PCR. CpG-DNA2 composed of two CpG-ODN monomers was prepared by enzyme cutting P-CpG-DNA.

• 采用质粒重组和PCR方法，将其串联成长为600多碱基对的DNA(P-CpG-DNA)，其中含有62个CpG基序，并将其中一部分用Sall酶切得到含两个CpG-DNA单体的CpG-DNA2。