AFB_1-BSA and donkey anti-mouse immunoglobulins were jet-positioned onto a nitrocellulose membrane which the distance was 4 mm.

• 将AFB_1-BSA(检测线)和驴抗鼠IgG(质控线)分别喷在硝酸纤维素膜(Nitrocellulose membrane，NCM)上，检测线和质控线两者之间相距4mm。