A colormetric method for the determination of potency in the fermentation broth was done as a dark blue material by reacting with anisaldehyde sulphric acid.

• 海南霉素在加热条件下能与茴香醛浓硫酸溶液反应产生深蓝色物质，在一定浓度范围内海南霉素效价与颜色深浅程度呈线性关系。