A C18 column and a mobile phase of 0. 05mol/L disodium hydrogen phosphate solution(adjusted to PH 6. 0 using phosphoric acid)-acetontrile(88: 12 ) were employed. The detective wave was 240urn.

• 采用C18柱，流动相为0.；05mol/L磷酸氢二钠溶液（用磷酸调至pH6