A 840bp DNA fragment containing dnrV gene in full length of 828bp was amplified from daunorubicin-producing strain Streptomyces coeruleorubidus SIPI-1482 by PCR,and then was subcloned into E.

• 通过PCR从柔红霉素产生菌天蓝淡红链霉菌SIPI-1482基因组DNA中扩增出约840bp的目的片段,其中包括了长度为828bp的dnrV全长序列。