3) Purified recombinant Ad plasmid DNA is digested with PacI to expose its inverted terminal repests( ITR), and then used to transfect AD-293 cells where deleted viral assembly genes are complemented in vivo.

• 3) 用线性化的重组腺病毒转染293细胞，获得基因组结构均一的重组腺病毒。